Human SLBP Recombinant Protein (N-His) (HA797012)

Name: Human SLBP Recombinant Protein (N-His)
Brand: abinScience
SKU: HA797012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HA797012
Description	Recombinant Human SLBP Protein, N-His (HA797012) expressed in E. coli, spanning Cys72-Asp198. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q14493
Protein length	Cys72-Asp198
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	17.47 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Histone RNA hairpin-binding protein; Histone stem-loop-binding protein; SLBP; HBP
Background	Histone RNA hairpin-binding protein is a ~31 kDa protein. RNA-binding protein involved in the histone pre-mRNA processing. Binds the stem-loop structure of replication-dependent histone pre-mRNAs and contributes to efficient 3'-end processing by stabilizing the complex between histone pre-mRNA and U7 small nuclear ribonucleoprotein (snRNP), via the histone downstream element (HDE). Plays an important role in targeting mature histone mRNA from the nucleus to the cytoplasm and to the translation machinery. Stabilizes mature histone mRNA and could be involved in cell-cycle regulation of histone gene expression. Involved in the mechanism by which growing oocytes accumulate histone proteins that support early embryogenesis. 1. Zheng, L. et al. (2003) Molecular and cellular biology 23, 1590-601. PMID: 12588979 2. Sullivan, KD. et al. (2009) RNA (New York, N.Y.) 15, 459-72. PMID: 19155325 3. Wang, ZF. et al. (1996) Genes & development 10, 3028-40. PMID: 8957003 4. Martin, F. et al. (1997) The EMBO journal 16, 769-78. PMID: 9049306
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant Human SLBP Protein, N-His

References

Datasheet

Human SLBP Recombinant Protein (N-His).pdf

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Formula

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Human SLBP Recombinant Protein (N-His) (HA797012)

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