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Human SLC26A3 Recombinant Protein (N-His) (HD023012)

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Overview
Catalog No.HD023012
Description
Recombinant Human SLC26A3 Protein, N-His (HD023012) expressed in E. coli, spanning Lys507-Phe764. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionP40879
Protein lengthLys507-Phe764
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 32.16 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesChloride anion exchanger, DRA, Down-regulated in adenoma, Protein DRA, SLC26A3, Solute carrier family 26 member 3
Background

Chloride anion exchanger is a ~84 kDa protein. Mediates chloride-bicarbonate exchange with a chloride bicarbonate stoichiometry of 2:1 in the intestinal epithelia. Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation.

1. Shcheynikov, N. et al. (2006) The Journal of general physiology 127, 511-24. PMID: 16606687
2. Hayashi, H. et al. (2009) American journal of physiology. Cell physiology 296, C1279-90. PMID: 19321737
3. Hayashi, H. et al. (2012) American journal of physiology. Cell physiology 302, C781-95. PMID: 22159084
4. Lee, JH. et al. (2012) Cellular signalling 24, 1821-30. PMID: 22627094
Note For research use only
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References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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