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Human SLC26A4 Recombinant Protein (N-His) (HB578012)

Human SLC26A4 Recombinant Protein (N-His)
Human SLC26A4 Recombinant Protein (N-His)
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Overview
Catalog No.HB578012
Description
Recombinant Human SLC26A4 Protein, N-His (HB578012) expressed in E. coli, spanning Ser517-Ser741. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionO43511
Protein lengthSer517-Ser741
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 27.79 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesPendrin; Sodium-independent chloride/iodide transporter; Solute carrier family 26 member 4; SLC26A4; PDS
Background

Pendrin is a ~85 kDa protein. Sodium-independent transporter of chloride and iodide. Mediates electroneutral chloride-bicarbonate, chloride-iodide and chloride-formate exchange with 1:1 stoichiometry. Mediates electroneutral iodide-bicarbonate exchange.

1. Scott, DA. et al. (1999) Nature genetics 21, 440-3. PMID: 10192399
2. Taylor, JP. et al. (2002) The Journal of clinical endocrinology and metabolism 87, 1778-84. PMID: 11932316
3. Yoshida, A. et al. (2002) The Journal of clinical endocrinology and metabolism 87, 3356-61. PMID: 12107249
4. Pfarr, N. et al. (2006) The Journal of clinical endocrinology and metabolism 91, 2678-81. PMID: 16684826
5. Chattaraj, P. et al. (2013) JAMA otolaryngology-- head & neck surgery 139, 907-13. PMID: 24051746
6. Scott, DA. et al. (2000) American journal of physiology. Cell physiology 278, C207-11. PMID: 10644529
7. Yoshida, A. et al. (2004) Endocrinology 145, 4301-8. PMID: 15155570
8. Xu, J. et al. (2022) Frontiers in molecular biosciences 9, 874186. PMID: 35601831
Note For research use only
Images
  • Human SLC26A4 Recombinant Protein (N-His)

    SDS-PAGE

    SDS-PAGE for Recombinant Human SLC26A4 Protein, N-His

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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