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Human SLC27A4 Recombinant Protein (N-His) (HV816022)

Human SLC27A4 Recombinant Protein (N-His)
Human SLC27A4 Recombinant Protein (N-His)
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Overview
Catalog No.HV816022
Description
Recombinant Human SLC27A4 Protein, N-His (HV816022) expressed in E. coli, spanning Thr75-Leu643. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionQ6P1M0
Protein lengthThr75-Leu643
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 65.91 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesLong-chain fatty acid transport protein 4; FATP-4; Fatty acid transport protein 4; Arachidonate--CoA ligase; 6.2.1.15; Long-chain-fatty-acid--CoA ligase; 6.2.1.3; Solute carrier family 27 member 4; Very long-chain acyl-CoA synthetase 4; ACSVL4; 6.2.1.-; SLC27A4; ACSVL4; FATP4
Background

Long-chain fatty acid transport protein 4 is a ~72 kDa protein. Mediates the levels of long-chain fatty acids (LCFA) in the cell by facilitating their transport across cell membranes. Appears to be the principal fatty acid transporter in small intestinal enterocytes. Also functions as an acyl-CoA ligase catalyzing the ATP-dependent formation of fatty acyl-CoA using LCFA and very-long-chain fatty acids (VLCFA) as substrates, which prevents fatty acid efflux from cells and might drive more fatty acid uptake. Plays a role in the formation of the epidermal barrier. Required for fat absorption in early embryogenesis.

1. Stahl, A. et al. (1999) Molecular cell 4, 299-308. PMID: 10518211
2. Gimeno, RE. et al. (2003) The Journal of biological chemistry 278, 16039-44. PMID: 12556534
3. Zhou, W. et al. (2010) Journal of biomolecular screening 15, 488-97. PMID: 20448275
4. Mitchell, RW. et al. (2011) Journal of neurochemistry 117, 735-46. PMID: 21395585
5. Krammer, J. et al. (2011) International journal of medical sciences 8, 599-614. PMID: 22022213
6. Ohkuni, A. et al. (2013) Biochemical and biophysical research communications 442, 195-201. PMID: 24269233
Note For research use only
Images
  • Human SLC27A4 Recombinant Protein (N-His)

    SDS-PAGE

    SDS-PAGE for Recombinant Human SLC27A4 Protein, N-His

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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