Human SLC39A4/ZIP4 Recombinant Protein (N-His) (HP316012)

Name: Human SLC39A4/ZIP4 Recombinant Protein (N-His)
Brand: abinScience
SKU: HP316012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP316012
Description	Recombinant Human SLC39A4/ZIP4 Protein, N-His (HP316012) expressed in E. coli, spanning Ser23-Tyr327. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q6P5W5
Protein length	Ser23-Tyr327
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Solute carrier family 39 member 4, Zinc transporter ZIP4, SLC39A4, Zrt- and Irt-like protein 4, ZIP-4, ZIP4
Background	Zinc transporter ZIP4 (SLC39A4) is a ~68 kDa protein. Selective transporter that mediates the uptake of Zn(2+). Plays an essential role for dietary zinc uptake from small intestine. The Zn(2+) uniporter activity is regulated by zinc availability. Also exhibits polyspecific binding and transport of Cu(2+), Cd(2+) and possibly Ni(2+) but at higher concentrations. _x005F_x005F_x000D_ _x005F_x005F_x000D_ 1. Mao, X. et al. (2007) The Journal of biological chemistry 282, 6992-7000. PMID: 17202136 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 2. Antala, S. et al. (2012) Biochemistry 51, 963-73. PMID: 22242765 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 3. Zhang, T. et al. (2016) Nature communications 7, 11979. PMID: 27321477 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 4. Zhang, T. et al. (2017) Science advances 3, e1700344. PMID: 28875161 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 5. Zhang, T. et al. (2019) The Biochemical journal 476, 1791-1803. PMID: 31164399 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 6. Zhang, T. et al. (2020) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34, 237-247. PMID: 31914589 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 7. Hoch, E. et al. (2020) International journal of molecular sciences 21. PMID: 31979155 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 8. Kuliyev, E. et al. (2021) The Journal of biological chemistry 296, 100269. PMID: 33837739 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 9. Liu, Y. et al. (2022) Scientific reports 12, 21083. PMID: 36473915 _x005F_x005F_x000D_
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human SLC39A4/ZIP4 Protein, N-His

References

Datasheet

Human SLC39A4/ZIP4 Recombinant Protein (N-His).pdf

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Human SLC39A4/ZIP4 Recombinant Protein (N-His) (HP316012)

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Datasheet

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