ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
35.28 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Sodium-coupled monocarboxylate transporter 1 (SLC5A8) is a ~66 kDa protein. Acts as an electrogenic sodium (Na(+)) and chloride (Cl-)-dependent sodium-coupled solute transporter, including transport of monocarboxylates (short-chain fatty acids including L-lactate, D-lactate, pyruvate, acetate, propionate, valerate and butyrate), mocarboxylate drugs (nicotinate, benzoate, salicylate and 5-aminosalicylate) and ketone bodies (beta-D-hydroxybutyrate, acetoacetate and alpha-ketoisocaproate), with a Na(+):substrate stoichiometry of between 4:1 and 2:1. Catalyzes passive carrier mediated diffusion of iodide. Mediates iodide transport from the thyrocyte into the colloid lumen through the apical membrane. May be responsible for the absorption of D-lactate and monocarboxylate drugs from the intestinal tract. Acts as a tumor suppressor, suppressing colony formation in colon cancer, prostate cancer and glioma cell lines.
1. Miyauchi, S. et al. (2004) The Journal of biological chemistry 279, 13293-6. PMID: 14966140
2. Coady, MJ. et al. (2004) The Journal of physiology 557, 719-31. PMID: 15090606
3. Itagaki, S. et al. (2006) Pharmaceutical research 23, 1209-16. PMID: 16729224
4. Martin, PM. et al. (2006) Journal of neurochemistry 98, 279-88. PMID: 16805814
5. Thangaraju, M. et al. (2006) Cancer research 66, 11560-4. PMID: 17178845
6. Gopal, E. et al. (2007) Pharmaceutical research 24, 575-84. PMID: 17245649
7. Coady, MJ. et al. (2007) Biophysical journal 93, 2325-31. PMID: 17526579
8. Miyauchi, S. et al. (2010) Biochimica et biophysica acta 1798, 1164-71. PMID: 20211600
9. Srivastava, S. et al. (2019) The journal of physiological sciences : JPS 69, 399-408. PMID: 30604288
10. Rodriguez, AM. et al. (2002) The Journal of clinical endocrinology and metabolism 87, 3500-3. PMID: 12107270
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human SLC5A8 Protein, N-GST & C-His
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