Human SMARCA4 Recombinant Protein (N-His) (HB550012)

Name: Human SMARCA4 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB550012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB550012
Description	Recombinant Human SMARCA4 Protein, N-His (HB550012) expressed in E. coli, spanning Leu988-Ser1209. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P51532
Protein length	Leu988-Ser1209
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	27.70 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Transcription activator BRG1; 3.6.4.-; ATP-dependent helicase SMARCA4; BRG1-associated factor 190A; BAF190A; Mitotic growth and transcription activator; Protein BRG-1; Protein brahma homolog 1; SNF2-beta; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4; SMARCA4; BAF190A; BRG1; SNF2B; SNF2L4
Background	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4 is a ~184 kDa protein. ATPase involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating the calcium-dependent release of a repressor complex and the recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by SMARCA4-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. 1. Liu, K. et al. (2004) Genes & development 18, 673-86. PMID: 15075294 2. Alpsoy, A. et al. (2018) The Journal of biological chemistry 293, 3892-3903. PMID: 29374058 3. Papillon, JPN. et al. (2018) Journal of medicinal chemistry 61, 10155-10172. PMID: 30339381 4. Zhu, X. et al. (2020) Nucleic acids research 48, 6563-6582. PMID: 32459350 5. Sánchez-Tilló, E. et al. (2010) Oncogene 29, 3490-500. PMID: 20418909
Note	For research use only

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SDS-PAGE for Recombinant Human SMARCA4 Protein, N-His

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Datasheet

Human SMARCA4 Recombinant Protein (N-His).pdf

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Human SMARCA4 Recombinant Protein (N-His) (HB550012)

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