Human SPI1 Recombinant Protein (N-GST & C-His) (HB273022)

Name: Human SPI1 Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HB273022
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB273022
Description	Recombinant Human SPI1 Protein, N-GST & C-His (HB273022) expressed in E. coli, spanning Arg171-His270. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P17947
Protein length	Arg171-His270
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	39.94 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	31 kDa-transforming protein, SPI1, Transcription factor PU.1
Background	Transcription factor PU.1 is a ~31 kDa protein. Pioneer transcription factor, which controls hematopoietic cell fate by decompacting stem cell heterochromatin and allowing other transcription factors to enter otherwise inaccessible genomic sites. Once in open chromatin, can directly control gene expression by binding genetic regulatory elements and can also more broadly influence transcription by recruiting transcription factors, such as interferon regulatory factors (IRFs), to otherwise inaccessible genomic regions. Transcriptionally activates genes important for myeloid and lymphoid lineages, such as CSF1R. Transcriptional activation from certain promoters, possibly containing low affinity binding sites, is achieved cooperatively with other transcription factors. FCER1A transactivation is achieved in cooperation with GATA1. 1. Pham, TH. et al. (2013) Nucleic acids research 41, 6391-402. PMID: 23658224 2. Le Coz, C. et al. (2021) The Journal of experimental medicine 218. PMID: 33951726
Note	For research use only

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Datasheet

Human SPI1 Recombinant Protein (N-GST & C-His).pdf

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Human SPI1 Recombinant Protein (N-GST & C-His) (HB273022)

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