Human SPTLC1 Recombinant Protein (N-His) (HC142012)

Name: Human SPTLC1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC142012
Price: 211 USD
Availability: InStock

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Catalog No.	HC142012
Description	Recombinant Human SPTLC1 Protein, N-His (HC142012) expressed in E. coli, spanning Ser90-Lys437. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O15269
Protein length	Ser90-Lys437
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	40.86 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	EC:2.3.1.50, LCB 1, LCB1, Long chain base biosynthesis protein 1, SPT 1, SPT1, SPTLC1, Serine palmitoyltransferase 1, Serine-palmitoyl-CoA transferase 1
Background	Serine palmitoyltransferase 1 is a ~52 kDa protein. Component of the serine palmitoyltransferase multisubunit enzyme (SPT) that catalyzes the initial and rate-limiting step in sphingolipid biosynthesis by condensing L-serine and activated acyl-CoA (most commonly palmitoyl-CoA) to form long-chain bases. The SPT complex is also composed of SPTLC2 or SPTLC3 and SPTSSA or SPTSSB. Within this complex, the heterodimer with SPTLC2 or SPTLC3 forms the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. 1. Han, G. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 8186-91. PMID: 19416851 2. Li, S. et al. (2021) Nature structural & molecular biology 28, 249-257. PMID: 33558762 3. Spears, ME. et al. (2022) Cell reports 40, 111415. PMID: 36170811 4. Hornemann, T. et al. (2009) The Journal of biological chemistry 284, 26322-30. PMID: 19648650 5. Wang, Y. et al. (2021) Nature structural & molecular biology 28, 240-248. PMID: 33558761
Note	For research use only

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Human SPTLC1 Recombinant Protein (N-His).pdf

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Human SPTLC1 Recombinant Protein (N-His) (HC142012)

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