Human SRCAP Recombinant Protein (N-His) (HP108012)

Name: Human SRCAP Recombinant Protein (N-His)
Brand: abinScience
SKU: HP108012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP108012
Description	_x005F_x000D_ _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ Recombinant Human SRCAP Protein, N-His (HP108012) expressed in E. coli, spanning Lys2044-Thr2197. Purity: >90% by SDS-PAGE. _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ Highlights _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ ●His-Tagged — N-terminal 6×His tag for IMAC purification. _x005F_x000D_ ●E. coli Expression — High-yield, cost-effective production. _x005F_x000D_ ●High Purity — >90% purity verified by SDS-PAGE. _x005F_x000D_ _x005F_x000D_ _x005F_x000D_
Expression system	E. coli
Accession	Q6ZRS2
Protein length	Lys2044-Thr2197
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	20.09 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Helicase SRCAP, 3.6.4.-, Domino homolog 2, Snf2-related CBP activator, SRCAP, KIAA0309
Background	Chromatin remodeling protein SRCAP is a ~343 kDa protein. Acts both as a chromatin remodeler and transcription coregulator. Catalytic component of the SRCAP complex which mediates the ATP-dependent exchange of histone H2AZ/H2B dimers for nucleosomal H2A/H2B, leading to transcriptional regulation of selected genes expression through chromatin remodeling. Acts as a coactivator for CREB-mediated transcription, steroid receptor-mediated transcription, and Notch-mediated transcription. _x005F_x000D_ _x005F_x000D_ 1. Ruhl, DD. et al. (2006) Biochemistry 45, 5671-7. PMID: 16634648 _x005F_x000D_ _x005F_x000D_ 2. Wong, MM. et al. (2007) The Journal of biological chemistry 282, 26132-9. PMID: 17617668 _x005F_x000D_ _x005F_x000D_ 3. Johnston, H. et al. (1999) The Journal of biological chemistry 274, 16370-6. PMID: 10347196 _x005F_x000D_ _x005F_x000D_ 4. Monroy, MA. et al. (2001) The Journal of biological chemistry 276, 40721-6. PMID: 11522779 _x005F_x000D_ _x005F_x000D_ 5. Monroy, MA. et al. (2003) Molecular endocrinology (Baltimore, Md.) 17, 2519-28. PMID: 14500758 _x005F_x000D_ _x005F_x000D_ 6. Eissenberg, JC. et al. (2005) Molecular and cellular biology 25, 6559-69. PMID: 16024792 _x005F_x000D_
Note	For research use only.

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SDS-PAGE for Recombinant Human SRCAP Protein, N-His

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Datasheet

Human SRCAP Recombinant Protein (N-His).pdf

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Human SRCAP Recombinant Protein (N-His) (HP108012)

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