Human SRSF3 Recombinant Protein (N-GST & C-His) (HB340012)

Name: Human SRSF3 Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HB340012
Price: 211 USD
Availability: InStock

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Catalog No.	HB340012
Description	Recombinant Human SRSF3 Protein, N-GST & C-His (HB340012) expressed in E. coli, spanning His2-Glu84. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P84103
Protein length	His2-Glu84
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	37.43 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Serine/arginine-rich splicing factor 3; Pre-mRNA-splicing factor SRP20; Splicing factor, arginine/serine-rich 3; SRSF3; SFRS3; SRP20
Background	Serine/arginine-rich splicing factor 3 is a ~19 kDa protein. Splicing factor, which binds the consensus motif 5'-C[ACU][AU]C[ACU][AC]C-3' within pre-mRNA and promotes specific exons inclusion during alternative splicing. Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites within exons. Also functions as an adapter involved in mRNA nuclear export. Binds mRNA which is thought to be transferred to the NXF1-NXT1 heterodimer for export (TAP/NXF1 pathway); enhances NXF1-NXT1 RNA-binding activity. Involved in nuclear export of m6A-containing mRNAs via interaction with YTHDC1: interaction with YTHDC1 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export. 1. Hargous, Y. et al. (2006) The EMBO journal 25, 5126-37. PMID: 17036044 2. Xiao, W. et al. (2016) Molecular cell 61, 507-519. PMID: 26876937 3. Meng, N. et al. (2020) Advanced science (Weinheim, Baden-Wurttemberg, Germany) 7, 1903233. PMID: 32440474 4. Huang, Y. et al. (2001) Molecular cell 7, 899-905. PMID: 11336712 5. Hautbergue, GM. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 5154-9. PMID: 18364396 6. Roundtree, IA. et al. (2017) eLife 6. PMID: 28984244
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant Human SRSF3 Protein, N-GST & C-His

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Datasheet

Human SRSF3 Recombinant Protein (N-GST & C-His).pdf

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Human SRSF3 Recombinant Protein (N-GST & C-His) (HB340012)

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