Human STARD3 Recombinant Protein (N-His) (HB422012)

Name: Human STARD3 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB422012
Price: 211 USD
Availability: InStock

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Catalog No.	HB422012
Description	Recombinant Human STARD3 Protein, N-His (HB422012) expressed in E. coli, spanning Ser232-Ala445. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q14849
Protein length	Ser232-Ala445
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	26.64 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	StAR-related lipid transfer protein 3; Metastatic lymph node gene 64 protein; MLN 64; Protein CAB1; START domain-containing protein 3; StARD3; STARD3; CAB1; MLN64
Background	StAR-related lipid transfer protein 3 is a ~50 kDa protein. Sterol-binding protein that mediates cholesterol transport from the endoplasmic reticulum to endosomes. The sterol transport mechanism is triggered by phosphorylation of FFAT motif that leads to membrane tethering between the endoplasmic reticulum and late endosomes via interaction with VAPA and VAPB. Acts as a lipid transfer protein that redirects sterol to the endosome at the expense of the cell membrane and favors membrane formation inside endosomes. May also mediate cholesterol transport between other membranes, such as mitochondria membrane or cell membrane. However, such results need additional experimental evidences; probably mainly mediates cholesterol transport from the endoplasmic reticulum to endosomes. 1. Alpy, F. et al. (2001) The Journal of biological chemistry 276, 4261-9. PMID: 11053434 2. Hölttä-Vuori, M. et al. (2005) Molecular biology of the cell 16, 3873-86. PMID: 15930133 3. Liapis, A. et al. (2012) PloS one 7, e34424. PMID: 22514632 4. Wilhelm, LP. et al. (2017) The EMBO journal 36, 1412-1433. PMID: 28377464 5. Di Mattia, T. et al. (2020) The EMBO journal 39, e104369. PMID: 33124732 6. Alpy, F. et al. (2013) Journal of cell science 126, 5500-12. PMID: 24105263 7. Zhang, M. et al. (2002) The Journal of biological chemistry 277, 33300-10. PMID: 12070139
Note	For research use only

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SDS-PAGE for Recombinant Human STARD3 Protein, N-His

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Datasheet

Human STARD3 Recombinant Protein (N-His).pdf

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Human STARD3 Recombinant Protein (N-His) (HB422012)

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