Human STIM1 Recombinant Protein (N-His) (HC687012)

Name: Human STIM1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC687012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC687012
Description	Recombinant Human STIM1 Protein, N-His (HC687012) expressed in E. coli, spanning Ser28-Leu211. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q13586
Protein length	Ser28-Leu211
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	23.37 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	STIM1, Stromal interaction molecule 1, GOK
Background	Stromal interaction molecule 1 (STIM1) is a ~77 kDa protein. Acts as a Ca(2+) sensor that gates two major inward rectifying Ca(2+) channels at the plasma membrane: Ca(2+) release-activated Ca(2+) (CRAC) channels and arachidonate-regulated Ca(2+)-selective (ARC) channels. Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates CRAC channel pore-forming subunits ORA1, ORA2 and ORAI3 to generate sustained and oscillatory Ca(2+) entry. Involved in enamel formation. 1. Roos, J. et al. (2005) The Journal of cell biology 169, 435-45. PMID: 15866891 2. Liou, J. et al. (2005) Current biology : CB 15, 1235-41. PMID: 16005298 3. Zhang, SL. et al. (2005) Nature 437, 902-5. PMID: 16208375 4. Spassova, MA. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 4040-5. PMID: 16537481 5. Peinelt, C. et al. (2006) Nature cell biology 8, 771-3. PMID: 16733527 6. Soboloff, J. et al. (2006) The Journal of biological chemistry 281, 20661-20665. PMID: 16766533 7. Mercer, JC. et al. (2006) The Journal of biological chemistry 281, 24979-90. PMID: 16807233 8. Stathopulos, PB. et al. (2008) Cell 135, 110-22. PMID: 18854159 9. Yuan, JP. et al. (2009) Nature cell biology 11, 337-43. PMID: 19182790 10. Park, CY. et al. (2009) Cell 136, 876-90. PMID: 19249086
Note	For research use only.

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SDS-PAGE for Recombinant Human STIM1 Protein, N-His

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Datasheet

Human STIM1 Recombinant Protein (N-His).pdf

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Human STIM1 Recombinant Protein (N-His) (HC687012)

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