Human SUMO1 Recombinant Protein (N-His) (HX224012)

Name: Human SUMO1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HX224012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HX224012
Description	Recombinant Human SUMO1 Protein, N-His (HX224012) expressed in E. coli, spanning Met1-Val101. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P63165
Protein length	Met1-Val101
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Predicted molecular weight	13.72 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	SUMO-1, SMT3C, SMT3H3, Sentrin, GMP1, Ubiquitin-like protein UBL1, UBL1, Small ubiquitin-related modifier 1, Ubiquitin-homology domain protein PIC1, SUMO1, Ubiquitin-like protein SMT3C, SMT3 homolog 3, Smt3C, GAP-modifying protein 1
Background	Small ubiquitin-related modifier 1 (SUMO1) is a ~11 kDa protein. Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalently attached to the voltage-gated potassium channel KCNB1; this modulates the gating characteristics of KCNB1. 1. Dai, XQ. et al. (2009) Journal of cell science 122, 775-9. PMID: 19223394 2. Sun, X. et al. (2014) PloS one 9, e92746. PMID: 24651376
Note	For research use only.

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SDS-PAGE for Recombinant Human SUMO1 Protein, N-His

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Datasheet

Human SUMO1 Recombinant Protein (N-His).pdf

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Human SUMO1 Recombinant Protein (N-His) (HX224012)

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