Human SUPT4H1 Recombinant Protein (N-His) (HD121012)

Name: Human SUPT4H1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HD121012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HD121012
Description	Recombinant Human SUPT4H1 Protein, N-His (HD121012) expressed in E. coli, spanning Met1-Thr117. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P63272
Protein length	Met1-Thr117
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	15.36 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	DRB sensitivity-inducing factor 14 kDa subunit, DRB sensitivity-inducing factor small subunit, DSIF p14, DSIF small subunit, SPT4H, SUPT4H, SUPT4H1, Transcription elongation factor SPT4, hSPT4
Background	Transcription elongation factor SPT4 is a ~13 kDa protein. Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. 1. Yamaguchi, Y. et al. (1999) The Journal of biological chemistry 274, 8085-92. PMID: 10075709 2. Yamaguchi, Y. et al. (1999) Cell 97, 41-51. PMID: 10199401 3. Kim, JB. et al. (1999) Molecular and cellular biology 19, 5960-8. PMID: 10454543 4. Wada, T. et al. (2000) Molecular cell 5, 1067-72. PMID: 10912001 5. Ping, YH. et al. (2001) The Journal of biological chemistry 276, 12951-8. PMID: 11112772 6. Renner, DB. et al. (2001) The Journal of biological chemistry 276, 42601-9. PMID: 11553615 7. Kim, DK. et al. (2003) Genes to cells : devoted to molecular & cellular mechanisms 8, 371-8. PMID: 12653964 8. Kwak, YT. et al. (2003) Molecular cell 11, 1055-66. PMID: 12718890 9. Mandal, SS. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 7572-7. PMID: 15136722 10. Jennings, BH. et al. (2004) Current biology : CB 14, 1680-4. PMID: 15380072
Note	For research use only

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Datasheet

Human SUPT4H1 Recombinant Protein (N-His).pdf

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Human SUPT4H1 Recombinant Protein (N-His) (HD121012)

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