ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
44.75 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Transcription elongation factor SPT5 is a ~121 kDa protein. Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II.
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2. Yamaguchi, Y. et al. (1999) Cell 97, 41-51. PMID: 10199401
3. Wen, Y. et al. (1999) Genes & development 13, 1774-9. PMID: 10421630
4. Ivanov, D. et al. (2000) Molecular and cellular biology 20, 2970-83. PMID: 10757782
5. Wada, T. et al. (2000) Molecular cell 5, 1067-72. PMID: 10912001
6. Ping, YH. et al. (2001) The Journal of biological chemistry 276, 12951-8. PMID: 11112772
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8. Kim, DK. et al. (2003) Genes to cells : devoted to molecular & cellular mechanisms 8, 371-8. PMID: 12653964
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