Human SYK Recombinant Protein (N-His) (HW747012)

Name: Human SYK Recombinant Protein (N-His)
Brand: abinScience
SKU: HW747012
Price: 211 USD
Availability: InStock

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Catalog No.	HW747012
Description	Recombinant Human SYK Protein, N-His (HW747012) expressed in E. coli, spanning Asp374-Asn635. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P43405
Protein length	Asp374-Asn635
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	32.58 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Spleen tyrosine kinase, SYK, Tyrosine-protein kinase SYK, p72-Syk
Background	Tyrosine-protein kinase SYK is a ~72 kDa protein. Non-receptor tyrosine kinase which mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR). Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development. Assembles into signaling complexes with activated receptors at the plasma membrane via interaction between its SH2 domains and the receptor tyrosine-phosphorylated ITAM domains. The association with the receptor can also be indirect and mediated by adapter proteins containing ITAM or partial hemITAM domains. The phosphorylation of the ITAM domains is generally mediated by SRC subfamily kinases upon engagement of the receptor. 1. Urzainqui, A. et al. (2002) Immunity 17, 401-12. PMID: 12387735 2. Wang, L. et al. (2021) Nature genetics 53, 500-510. PMID: 33782605 3. Chiu, CW. et al. (2002) The EMBO journal 21, 6461-72. PMID: 12456653 4. Shim, EK. et al. (2004) FEBS letters 575, 35-40. PMID: 15388330 5. M Gagné, L. et al. (2021) The Journal of biological chemistry 297, 101291. PMID: 34634301 6. Law, CL. et al. (1996) Molecular and cellular biology 16, 1305-15. PMID: 8657103 7. Ratthé, C. et al. (2004) Journal of leukocyte biology 76, 162-8. PMID: 15123770
Note	For research use only.

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SDS-PAGE for Human SYK Recombinant Protein (N-His)

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HW747012-SDS-PAGE-1.jpg

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Human SYK Recombinant Protein (N-His).pdf

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Human SYK Recombinant Protein (N-His) (HW747012)

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