Human TAP2 Recombinant Protein (N-His) (HA791012)

Name: Human TAP2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HA791012
Price: 211 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug
1mg

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	HA791012
Description	Recombinant Human TAP2 Protein, N-His (HA791012) expressed in E. coli, spanning Leu464-Leu686. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q03519
Protein length	Leu464-Leu686
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	26.69 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Antigen peptide transporter 2; APT2; 7.4.2.14; ATP-binding cassette sub-family B member 3; Peptide supply factor 2; Peptide transporter PSF2; PSF-2; Peptide transporter TAP2; Peptide transporter involved in antigen processing 2; Really interesting new gene 11 protein; RING11; TAP2; ABCB3; PSF2; RING11; Y1
Background	Antigen peptide transporter 2 is a ~75 kDa protein. ABC transporter associated with antigen processing. In complex with TAP1 mediates unidirectional translocation of peptide antigens from cytosol to endoplasmic reticulum (ER) for loading onto MHC class I (MHCI) molecules. Uses the chemical energy of ATP to export peptides against the concentration gradient. During the transport cycle alternates between 'inward-facing' state with peptide binding site facing the cytosol to 'outward-facing' state with peptide binding site facing the ER lumen. Peptide antigen binding to ATP-loaded TAP1-TAP2 induces a switch to hydrolysis-competent 'outward-facing' conformation ready for peptide loading onto nascent MHCI molecules. 1. Grossmann, N. et al. (2014) Nature communications 5, 5419. PMID: 25377891 2. Fischbach, H. et al. (2015) Nature communications 6, 6199. PMID: 25656091 3. Gorbulev, S. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 3732-7. PMID: 11274390 4. van Endert, PM. et al. (1995) The Journal of experimental medicine 182, 1883-95. PMID: 7500034 5. Uebel, S. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 8976-81. PMID: 9256420 6. Kelly, A. et al. (1992) Nature 355, 641-4. PMID: 1538751
Note	For research use only

Images

SDS-PAGE

SDS-PAGE for Recombinant Human TAP2 Protein, N-His

References

Datasheet

Human TAP2 Recombinant Protein (N-His).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Human TAP2 Recombinant Protein (N-His) (HA791012)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us