Human TAX1BP1 Recombinant Protein (N-His) (HP605012)

Name: Human TAX1BP1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HP605012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP605012
Description	Recombinant Human TAX1BP1 Protein, N-His (HP605012) expressed in E. coli, spanning His16-Lys146. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q86VP1
Protein length	His16-Lys146
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	17.41 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Tax1-binding protein 1, TAX1BP1, TRAF6-binding protein, T6BP
Background	Tax1-binding protein 1 (TAX1BP1) is a ~90 kDa protein. Ubiquitin-binding adapter that participates in inflammatory, antiviral and innate immune processes as well as selective autophagy regulation. Plays a key role in the negative regulation of NF-kappa-B and IRF3 signalings by acting as an adapter for the ubiquitin-editing enzyme A20/TNFAIP3 to bind and inactivate its substrates. Disrupts the interactions between the E3 ubiquitin ligase TRAF3 and TBK1/IKBKE to attenuate 'Lys63'-linked polyubiquitination of TBK1 and thereby IFN-beta production. Also recruits A20/TNFAIP3 to ubiquitinated signaling proteins TRAF6 and RIPK1, leading to their deubiquitination and disruption of IL-1 and TNF-induced NF-kappa-B signaling pathways. Inhibits virus-induced apoptosis by inducing the 'Lys-48'-linked polyubiquitination and degradation of MAVS via recruitment of the E3 ligase ITCH, thereby attenuating MAVS-mediated apoptosis signaling. 1. Hu, S. et al. (2018) Journal of molecular biology 430, 3283-3296. PMID: 29940186 2. Fu, T. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E11651-E11660. PMID: 30459273 3. Baillet, N. et al. (2019) Viruses 11. PMID: 30909570 4. Shembade, N. et al. (2007) The EMBO journal 26, 3910-22. PMID: 17703191 5. Gao, L. et al. (2011) The Journal of biological chemistry 286, 36592-602. PMID: 21885437 6. Choi, YB. et al. (2017) Molecular and cellular biology 37. PMID: 27736772 7. Tumbarello, DA. et al. (2015) PLoS pathogens 11, e1005174. PMID: 26451915 8. Ohnstad, AE. et al. (2020) The EMBO journal 39, e104948. PMID: 33226137 9. Turco, E. et al. (2021) Nature communications 12, 5212. PMID: 34471133 10. Yang, Q. et al. (2017) PLoS pathogens 13, e1006600. PMID: 28898289
Note	For research use only.

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SDS-PAGE for Recombinant Human TAX1BP1 Protein, N-His

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Datasheet

Human TAX1BP1 Recombinant Protein (N-His).pdf

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Human TAX1BP1 Recombinant Protein (N-His) (HP605012)

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