Human TDP2 Recombinant Protein (N-His) (HF005012)

Name: Human TDP2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HF005012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HF005012
Description	Recombinant Human TDP2 Protein, N-His (HF005012) expressed in E. coli, spanning Gly111-Leu362. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O95551
Protein length	Gly111-Leu362
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	30.96 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Tyr-DNA phosphodiesterase 2, TDP2, hTDP2, 5'-tyrosyl-DNA phosphodiesterase, Tyrosyl-DNA phosphodiesterase 2, EAP2, Tyrosyl-RNA phosphodiesterase, EAPII, TTRAP, VPg unlinkase, 5'-Tyr-DNA phosphodiesterase, TRAF and TNF receptor-associated protein, ETS1-associated protein II, ETS1-associated protein 2
Background	Tyrosyl-DNA phosphodiesterase 2 (TDP2) is a ~40 kDa protein. DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DNA double-strand breaks/DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Thereby, protects the transcription of many genes involved in neurological development and maintenance from the abortive activity of TOP2. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DSBs due to DNA damage by radiation and free radicals. 1. Schellenberg, MJ. et al. (2016) Nucleic acids research 44, 3829-44. PMID: 27060144 2. Hornyak, P. et al. (2016) The Biochemical journal 473, 1869-79. PMID: 27099339
Note	For research use only.

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SDS-PAGE for Recombinant Human TDP2 Protein, N-His

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Datasheet

Human TDP2 Recombinant Protein (N-His).pdf

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Human TDP2 Recombinant Protein (N-His) (HF005012)

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