Human TET3 Recombinant Protein (N-His) (HC899012)

Name: Human TET3 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC899012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HC899012
Description	Recombinant Human TET3 Protein, N-His (HC899012) expressed in E. coli, spanning Val835-Gln1140. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O43151
Protein length	Val835-Gln1140
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	36.61 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	CXXC10, EC:1.14.11.80, KIAA0401, Methylcytosine dioxygenase TET3, TET3
Background	Methylcytosine dioxygenase TET3 is a ~193 kDa protein. Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Selectively binds to the promoter region of target genes and contributes to regulate the expression of numerous developmental genes. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. 1. Beck, DB. et al. (2020) American journal of human genetics 106, 234-245. PMID: 31928709 2. Xu, Y. et al. (2012) Cell 151, 1200-13. PMID: 23217707 3. Deplus, R. et al. (2013) The EMBO journal 32, 645-55. PMID: 23353889 4. Xu, C. et al. (2018) Structure (London, England : 1993) 26, 85-95.e3. PMID: 29276034
Note	For research use only

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Datasheet

Human TET3 Recombinant Protein (N-His).pdf

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Human TET3 Recombinant Protein (N-His) (HC899012)

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