Human TOMM20 Recombinant Protein (N-GST & C-His) (HS890012)

Name: Human TOMM20 Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HS890012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HS890012
Description	Recombinant Human TOMM20 Protein, N-GST & C-His (HS890012) expressed in E. coli, spanning Arg51-Glu145. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q15388
Protein length	Arg51-Glu145
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	38.66 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Mitochondrial 20 kDa outer membrane protein, Mitochondrial import receptor subunit TOM20 homolog, KIAA0016, TOMM20, Outer mitochondrial membrane receptor Tom20
Background	Mitochondrial import receptor subunit TOM20 homolog (TOMM20) is a ~16 kDa protein. Central receptor component of the translocase of the outer membrane of mitochondria (TOM) complex essential for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOMM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. The TOM complex associates with the ion channel VDAC2 and PINK1 kinase at depolarized mitochondria, this interaction stabilizes PINK1 at the outer mitochondrial membrane and triggers downstream mitophagy by the recruitment of the E3 ubiquitin ligase PRKN. Required for the translocation across the mitochondrial outer membrane of cytochrome P450 monooxygenases. 1. Callegari, S. et al. (2025) Science (New York, N.Y.) 388, 303-310. PMID: 40080546 2. Kato, H. et al. (2008) Biochemical and biophysical research communications 369, 958-63. PMID: 18331822
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human TOMM20 Protein, N-GST & C-His

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Datasheet

Human TOMM20 Recombinant Protein (N-GST & C-His).pdf

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Human TOMM20 Recombinant Protein (N-GST & C-His) (HS890012)

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