Human TOP2A Recombinant Protein (N-His) (HY175012)

Name: Human TOP2A Recombinant Protein (N-His)
Brand: abinScience
SKU: HY175012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HY175012
Description	Recombinant Human TOP2A Protein, N-His (HY175012) expressed in E. coli, spanning Ile923-Arg1148. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P11388
Protein length	Ile923-Arg1148
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	28.79 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	DNA topoisomerase II, alpha isozyme, TOP2A, DNA topoisomerase 2-alpha, TOP2
Background	DNA topoisomerase 2-alpha (TOP2A) is a ~174 kDa protein. Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand. May play a role in regulating the period length of BMAL1 transcriptional oscillation. 1. Wyles, JP. et al. (2007) Nucleic acids research 35, 4076-85. PMID: 17567603 2. Williamson, EA. et al. (2008) Nucleic acids research 36, 5822-31. PMID: 18790802 3. Ramamoorthy, M. et al. (2012) Nucleic acids research 40, 1621-35. PMID: 22013166 4. Lee, S. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 2925-30. PMID: 22323612 5. Kratz, AS. et al. (2016) Cell cycle (Georgetown, Tex.) 15, 3419-3431. PMID: 27754753
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human TOP2A Protein, N-His

References

HY175012-SDS-PAGE-1.jpg

Datasheet

Human TOP2A Recombinant Protein (N-His).pdf

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Human TOP2A Recombinant Protein (N-His) (HY175012)

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