Human 5T4 Recombinant Protein (C-His) (HC281011)

Name: Human 5T4 Recombinant Protein (C-His)
Brand: abinScience
SKU: HC281011
Price: 298 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.	HC281011
Description	Recombinant Human TPBG/5T4 Protein, C-His (HC281011) expressed in Mammalian Cells, spanning Asp60-Asp345. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian cells
Accession	Q13641
Protein length	Asp60-Asp345
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Predicted molecular weight	34.54 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	5T4, TPBG, WAIF1, M6P1, 5T4 oncofetal trophoblast glycoprotein, Trophoblast glycoprotein, 5T4 oncofetal antigen, 5T4 oncotrophoblast glycoprotein, Wnt-activated inhibitory factor 1
Background	Trophoblast glycoprotein (TPBG/5T4) is a ~46 kDa protein. May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.
Note	For research use only.

Images

Bioactivity

Detects Human TPBG/5T4 Antibody (SAA0571) in indirect ELISAs.
Bioactivity

Detected by Research Grade Naptumomab (Cat No.: HC281016) in indirect ELISA.
Bioactivity

Detected by Research Grade Anti-Human TPBG/5T4 (PF-06263507) (Cat No.: HC281026) in indirect ELISA.
Bioactivity

Detected by Research Grade Anti-Human TPBG/5T4 Antibody (825c) (Cat No.: HC281076) in indirect ELISA.
Bioactivity

Detected by Anti-Human TPBG/5T4 Antibody (SAA0571) (Cat No.: HC281207) in indirect ELISA.
SDS-PAGE

SDS-PAGE for Recombinant Human TPBG/5T4 Protein, C-His.