Human UBA5 Recombinant Protein (N-His) (HD758012)

Name: Human UBA5 Recombinant Protein (N-His)
Brand: abinScience
SKU: HD758012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HD758012
Description	Recombinant Human UBA5 Protein, N-His (HD758012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE.. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% as determined by SDS-PAGE.
Expression system	E. coli
Accession	Q9GZZ9
Protein length	Lys70-Asn286
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	ThiFP1, UBA5, UBE1DC1, UFM1-activating enzyme, Ubiquitin-activating enzyme 5, Ubiquitin-activating enzyme E1 domain-containing protein 1, Ubiquitin-like modifier-activating enzyme 5
Background	Ubiquitin-like modifier-activating enzyme 5 is a ~44 kDa protein. E1-like enzyme which specifically catalyzes the first step in ufmylation. Activates UFM1 by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a UFM1-E1 thioester and free AMP. Activates UFM1 via a trans-binding mechanism, in which UFM1 interacts with distinct sites in both subunits of the UBA5 homodimer. Trans-binding also promotes stabilization of the UBA5 homodimer, and enhances ATP-binding. Transfer of UFM1 from UBA5 to the E2-like enzyme UFC1 also takes place using a trans mechanism. 1. Komatsu, M. et al. (2004) The EMBO journal 23, 1977-86. PMID: 15071506 2. Zheng, M. et al. (2008) Journal of cellular biochemistry 104, 2324-34. PMID: 18442052 3. Bacik, JP. et al. (2010) The Journal of biological chemistry 285, 20273-80. PMID: 20368332 4. Yoo, HM. et al. (2014) Molecular cell 56, 261-274. PMID: 25219498 5. Habisov, S. et al. (2016) The Journal of biological chemistry 291, 9025-41. PMID: 26929408 6. Muona, M. et al. (2016) American journal of human genetics 99, 683-694. PMID: 27545674 7. Colin, E. et al. (2016) American journal of human genetics 99, 695-703. PMID: 27545681 8. Oweis, W. et al. (2016) Cell reports 16, 3113-3120. PMID: 27653677 9. Soudah, N. et al. (2019) Journal of molecular biology 431, 463-478. PMID: 30412706 10. Walczak, CP. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 1299-1308. PMID: 30626644
Note	For research use only

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Datasheet

Human UBA5 Recombinant Protein (N-His).pdf

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Human UBA5 Recombinant Protein (N-His) (HD758012)

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