Human UBE3C Recombinant Protein (N-His) (HD238012)

Name: Human UBE3C Recombinant Protein (N-His)
Brand: abinScience
SKU: HD238012
Price: 211 USD
Availability: InStock

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Catalog No.	HD238012
Description	Recombinant Human UBE3C Protein, N-His (HD238012) expressed in E. coli, spanning Phe719-Ser1083. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q15386
Protein length	Phe719-Ser1083
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	43.64 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	EC:2.3.2.26, HECT-type ubiquitin transferase E3C, HectH2, Homologous to E6AP carboxyl terminus homologous protein 2, KIAA0010, KIAA10, RAUL, RTA-associated ubiquitin ligase, UBE3C, Ubiquitin-protein ligase E3C
Background	Ubiquitin-protein ligase E3C is a ~123 kDa protein. E3 ubiquitin-protein ligase that specifically catalyzes 'Lys-29'- and 'Lys-48'-linked polyubiquitin chains. Accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Associates with the proteasome and promotes elongation of ubiquitin chains on substrates bound to the 26S proteasome. Also catalyzes 'Lys-29'- and 'Lys-48'-linked ubiquitination of 26S proteasome subunit ADRM1/RPN13 in response to proteotoxic stress, impairing the ability of the proteasome to bind and degrade ubiquitin-conjugated proteins. Acts as a negative regulator of autophagy by mediating 'Lys-29'- and 'Lys-48'-linked ubiquitination of PIK3C3/VPS34, promoting its degradation. 1. You, J. et al. (2001) The Journal of biological chemistry 276, 19871-8. PMID: 11278995 2. You, J. et al. (2003) The Journal of biological chemistry 278, 23369-75. PMID: 12692129 3. Wang, M. et al. (2005) The EMBO journal 24, 4324-33. PMID: 16341092 4. Wang, M. et al. (2006) The EMBO journal 25, 1710-9. PMID: 16601690 5. Chu, BW. et al. (2013) The Journal of biological chemistry 288, 34575-87. PMID: 24158444 6. Besche, HC. et al. (2014) The EMBO journal 33, 1159-76. PMID: 24811749 7. Kristariyanto, YA. et al. (2015) Molecular cell 58, 83-94. PMID: 25752573 8. Michel, MA. et al. (2015) Molecular cell 58, 95-109. PMID: 25752577 9. Singh, S. et al. (2020) The Biochemical journal 477, 905-923. PMID: 32039437 10. Chen, YH. et al. (2021) Nature communications 12, 1322. PMID: 33637724
Note	For research use only

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Human UBE3C Recombinant Protein (N-His).pdf

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Human UBE3C Recombinant Protein (N-His) (HD238012)

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