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Human UGT1A9 Recombinant Protein (N-His) (HC949012)

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Overview
Catalog No.HC949012
Description
Recombinant Human UGT1A9 Protein, N-His (HC949012) expressed in E. coli, spanning Gly26-Ile275. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionO60656
Protein lengthGly26-Ile275
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 31.16 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesEC:2.4.1.17, GNT1, UDP-glucuronosyltransferase 1-9, UDP-glucuronosyltransferase 1-I, UDP-glucuronosyltransferase 1A9, UDPGT 1-9, UGT-1I, UGT1, UGT1*9, UGT1-09, UGT1.9, UGT1A9, UGT1I, lugP4
Background

UDP-glucuronosyltransferase 1A9 is a ~59 kDa protein. UDP-glucuronosyltransferase (UGT) that catalyzes phase II biotransformation reactions in which lipophilic substrates are conjugated with glucuronic acid to increase the metabolite's water solubility, thereby facilitating excretion into either the urine or bile. Essential for the elimination and detoxification of drugs, xenobiotics and endogenous compounds. Catalyzes the glucuronidation of endogenous estrogen hormones such as estradiol and estrone. Involved in the glucuronidation of arachidonic acid (AA) and AA-derived eicosanoids including 15-HETE, PGB1 and F2-isoprostanes (8-iso-PGF2alpha and 5-epi-5-F2t-IsoP). Glucuronates the phytochemical ferulic acid efficently at both the phenolic or the carboxylic acid group.

1. Gagné, JF. et al. (2002) Molecular pharmacology 62, 608-17. PMID: 12181437
2. Picard, N. et al. (2005) Drug metabolism and disposition: the biological fate of chemicals 33, 139-46. PMID: 15470161
3. Lépine, J. et al. (2004) The Journal of clinical endocrinology and metabolism 89, 5222-32. PMID: 15472229
4. Girard, H. et al. (2007) Pharmacogenetics and genomics 17, 1077-89. PMID: 18004212
5. Joseph, TB. et al. (2007) Molecular pharmaceutics 4, 883-94. PMID: 18052087
6. Alonen, A. et al. (2008) Biochemical pharmacology 76, 763-72. PMID: 18674515
7. Tang, L. et al. (2009) Molecular pharmaceutics 6, 1466-82. PMID: 19545173
8. Little, JM. et al. (2004) Journal of lipid research 45, 1694-703. PMID: 15231852
9. Li, X. et al. (2011) Drug metabolism and pharmacokinetics 26, 341-50. PMID: 21422672
10. Milne, GL. et al. (2024) Redox biology 70, 103020. PMID: 38211441
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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