Human USP37 Recombinant Protein (N-His) (HA158012)

Name: Human USP37 Recombinant Protein (N-His)
Brand: abinScience
SKU: HA158012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HA158012
Description	Recombinant Human USP37 Protein, N-His (HA158012) expressed in E. coli, spanning Met1-Arg106. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q86T82
Protein length	Met1-Arg106
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	14.26 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Ubiquitin carboxyl-terminal hydrolase 37, 3.4.19.12, Deubiquitinating enzyme 37, Ubiquitin thioesterase 37, Ubiquitin-specific-processing protease 37, USP37, KIAA1594
Background	Ubiquitin carboxyl-terminal hydrolase 37 (USP37) is a ~110 kDa protein. Deubiquitinase that plays a role in different processes including cell cycle regulation, DNA replication or DNA damage response. Antagonizes the anaphase-promoting complex (APC/C) during G1/S transition by mediating deubiquitination of cyclin-A (CCNA1 and CCNA2), thereby promoting S phase entry. Specifically mediates deubiquitination of 'Lys-11'-linked polyubiquitin chains, a specific ubiquitin-linkage type mediated by the APC/C complex. Phosphorylation at Ser-628 during G1/S phase maximizes the deubiquitinase activity, leading to prevent degradation of cyclin-A (CCNA1 and CCNA2). Plays an important role in the regulation of DNA replication by stabilizing the licensing factor CDT1. 1. Yeh, C. et al. (2015) Current biology : CB 25, 2290-9. PMID: 26299517 2. Hernández-Pérez, S. et al. (2016) Molecular oncology 10, 1196-206. PMID: 27296872 3. Xiao, Z. et al. (2019) American journal of cancer research 9, 2749-2759. PMID: 31911859 4. Stromberg, BR. et al. (2021) The Journal of biological chemistry 297, 101184. PMID: 34509474 5. Huang, X. et al. (2011) Molecular cell 42, 511-23. PMID: 21596315 6. Wu, C. et al. (2021) Nucleic acids research 49, 11224-11240. PMID: 34606619
Note	For research use only.

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SDS-PAGE for Recombinant Human USP37 Protein, N-His

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Datasheet

Human USP37 Recombinant Protein (N-His).pdf

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Human USP37 Recombinant Protein (N-His) (HA158012)

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