ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
50.85 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Ubiquitin carboxyl-terminal hydrolase 9X is a ~290 kDa protein. Deubiquitinase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. May therefore play an important regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. Specifically hydrolyzes 'Lys-11'-, followed by 'Lys-63'-, 'Lys-48'- and 'Lys-6'-linked polyubiquitins chains. Essential component of TGF-beta/BMP signaling cascade. Specifically deubiquitinates monoubiquitinated SMAD4, opposing the activity of E3 ubiquitin-protein ligase TRIM33.
1. Al-Hakim, AK. et al. (2008) The Biochemical journal 411, 249-60. PMID: 18254724
2. Dupont, S. et al. (2009) Cell 136, 123-35. PMID: 19135894
3. Grou, CP. et al. (2012) The Journal of biological chemistry 287, 12815-27. PMID: 22371489
4. Zhao, Y. et al. (2015) The EMBO journal 34, 1687-703. PMID: 25944111
5. Zhang, Y. et al. (2018) The Biochemical journal 475, 1507-1522. PMID: 29626158
6. Paudel, P. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 7288-7297. PMID: 30914461
7. Wang, P. et al. (2023) The Journal of biological chemistry 299, 105055. PMID: 37454738
9. Vong, QP. et al. (2005) Science (New York, N.Y.) 310, 1499-504. PMID: 16322459
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