ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
32.93 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, hVDAC1, Porin 31HL, Porin 31HM, Voltage-dependent anion-selective channel protein 1, VDAC1, VDAC-1, VDAC
Background
Non-selective voltage-gated ion channel VDAC1 is a ~30 kDa protein. Non-selective voltage-gated ion channel that mediates the transport of anions and cations through the mitochondrion outer membrane and plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. Binds various signaling molecules, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterols cholesterol and oxysterol.
1. Stadtmüller, U. et al. (1999) Biological chemistry 380, 1461-6. PMID: 10661876
2. Thinnes, FP. et al. (2001) Pflugers Archiv : European journal of physiology 443 Suppl 1, S111-6. PMID: 11845315
3. Hiller, S. et al. (2008) Science (New York, N.Y.) 321, 1206-10. PMID: 18755977
4. Marginedas-Freixa, I. et al. (2018) Scientific reports 8, 11384. PMID: 30061676
5. Blachly-Dyson, E. et al. (1993) The Journal of biological chemistry 268, 1835-41. PMID: 8420959
6. Dadsena, S. et al. (2019) Nature communications 10, 1832. PMID: 31015432
7. Ham, SJ. et al. (2020) Proceedings of the National Academy of Sciences of the United States of America 117, 4281-4291. PMID: 32047033
10. Chen, Y. et al. (2010) Biochemical and biophysical research communications 394, 798-803. PMID: 20230784
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human VDAC1 Protein, N-His
Bioactivity
Detected by Anti-VDAC1 Antibody (n152b/11) (Cat No.: HB031013) in indirect ELISA.
Western blot
Detected by Anti-VDAC1 monoclonal antibody (Catalog No: HB031013)
中文
English
한국어
日本語
Español
Français
Русский
