Human VPS26A Recombinant Protein (N-His) (HB599012)

Name: Human VPS26A Recombinant Protein (N-His)
Brand: abinScience
SKU: HB599012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HB599012
Description	Recombinant Human VPS26A Protein, N-His (HB599012) expressed in E. coli, spanning Met1-Gln305. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O75436
Protein length	Met1-Gln305
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	37.74 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Vacuolar protein sorting-associated protein 26A; Vesicle protein sorting 26A; hVPS26; VPS26A; VPS26
Background	Vacuolar protein sorting-associated protein 26A is a ~38 kDa protein. Acts as a component of the retromer cargo-selective complex (CSC). The CSC is believed to be the core functional component of retromer or respective retromer complex variants acting to prevent missorting of selected transmembrane cargo proteins into the lysosomal degradation pathway. The recruitment of the CSC to the endosomal membrane involves RAB7A and SNX3. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX3-retromer mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway. 1. Seaman, MN. (2004) The Journal of cell biology 165, 111-22. PMID: 15078902 2. Arighi, CN. et al. (2004) The Journal of cell biology 165, 123-33. PMID: 15078903 3. Vergés, M. et al. (2004) Nature cell biology 6, 763-9. PMID: 15247922 4. Harbour, ME. et al. (2012) The Biochemical journal 442, 209-20. PMID: 22070227 5. Harbour, ME. et al. (2010) Journal of cell science 123, 3703-17. PMID: 20923837 6. Fjorback, AW. et al. (2012) The Journal of neuroscience : the official journal of the Society for Neuroscience 32, 1467-80. PMID: 22279231 7. Gullapalli, A. et al. (2006) Molecular biology of the cell 17, 1228-38. PMID: 16407403 8. Temkin, P. et al. (2011) Nature cell biology 13, 715-21. PMID: 21602791
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant Human VPS26A Protein, N-His

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Datasheet

Human VPS26A Recombinant Protein (N-His).pdf

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Human VPS26A Recombinant Protein (N-His) (HB599012)

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