Human WDR48 Recombinant Protein (N-His) (HP117012)

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Overview

Catalog No.	HP117012
Description	Recombinant Human WDR48 Protein, N-His (HP117012) expressed in E. coli, spanning Gly32-Lys358. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q8TAF3
Protein length	Gly32-Lys358
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	USP1-associated factor 1, WD repeat-containing protein 48, KIAA1449, WDR48, p80, WD repeat endosomal protein, UAF1
Background	WD repeat-containing protein 48 (WDR48) is a ~76 kDa protein. Regulator of deubiquitinating complexes, which acts as a strong activator of USP1, USP12 and USP46. Enhances the USP1-mediated deubiquitination of FANCD2; USP1 being almost inactive by itself. Activates deubiquitination by increasing the catalytic turnover without increasing the affinity of deubiquitinating enzymes for the substrate. Also activates deubiquitinating activity of complexes containing USP12. In complex with USP12, acts as a potential tumor suppressor by positively regulating PHLPP1 stability. 1. Cohn, MA. et al. (2007) Molecular cell 28, 786-97. PMID: 18082604 2. Cohn, MA. et al. (2009) The Journal of biological chemistry 284, 5343-51. PMID: 19075014 3. Yin, J. et al. (2015) Structure (London, England : 1993) 23, 2043-54. PMID: 26388029 4. Liang, F. et al. (2019) Nature communications 10, 2849. PMID: 31253762 5. Li, H. et al. (2016) Molecular cell 63, 249-260. PMID: 27373336 7. Gangula, NR. et al. (2013) The Journal of biological chemistry 288, 34545-54. PMID: 24145035
Note	For research use only.

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SDS-PAGE for Recombinant Human WDR48 Protein, N-His

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HP117012-SDS-PAGE-1.jpg

Datasheet

Human WDR48 Recombinant Protein (N-His).pdf

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Human WDR48 Recombinant Protein (N-His) (HP117012)

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