Human WIPI2 Recombinant Protein (N-His) (HD733012)

Name: Human WIPI2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HD733012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HD733012
Description	Recombinant Human WIPI2 Protein, N-His (HD733012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE.. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% as determined by SDS-PAGE.
Expression system	E. coli
Accession	Q9Y4P8
Protein length	Met1-Arg374
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	WD repeat domain phosphoinositide-interacting protein 2, WIPI-2, WIPI2, WIPI49-like protein 2
Background	WD repeat domain phosphoinositide-interacting protein 2 is a ~49 kDa protein. Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Involved in an early step of the formation of preautophagosomal structures. Binds and is activated by phosphatidylinositol 3-phosphate (PtdIns3P) forming on membranes of the endoplasmic reticulum upon activation of the upstream ULK1 and PI3 kinases. Mediates ER-isolation membranes contacts by interacting with the ULK1:RB1CC1 complex and PtdIns3P. Once activated, WIPI2 recruits at phagophore assembly sites the ATG12-ATG5-ATG16L1 complex that directly controls the elongation of the nascent autophagosomal membrane. 1. Polson, HE. et al. (2010) Autophagy 6, 506-22. PMID: 20505359 2. Bakula, D. et al. (2017) Nature communications 8, 15637. PMID: 28561066 3. Zhao, YG. et al. (2017) Molecular cell 67, 974-989.e6. PMID: 28890335
Note	For research use only

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Datasheet

Human WIPI2 Recombinant Protein (N-His).pdf

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Human WIPI2 Recombinant Protein (N-His) (HD733012)

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