Human WWC1 Recombinant Protein (N-His) (HV200012)

Name: Human WWC1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HV200012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HV200012
Description	Recombinant Human WWC1 Protein, N-His (HV200012) expressed in E. coli, spanning Glu655-Val1119. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q8IX03
Protein length	Glu655-Val1119
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	55.24 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Kidney and brain protein, WWC1, Protein KIBRA, KIBRA, WW domain-containing protein 1, HBeAg-binding protein 3, KIAA0869
Background	Protein KIBRA (WWC1) is a ~125 kDa protein. Regulator of the Hippo signaling pathway, also known as the Salvador-Warts-Hippo (SWH) pathway. Enhances phosphorylation of LATS1 and YAP1 and negatively regulates cell proliferation and organ growth due to a suppression of the transcriptional activity of YAP1, the major effector of the Hippo pathway. Along with NF2 can synergistically induce the phosphorylation of LATS1 and LATS2 and function in the regulation of Hippo signaling pathway. Acts as a transcriptional coactivator of ESR1 which plays an essential role in DYNLL1-mediated ESR1 transactivation. Regulates collagen-stimulated activation of the ERK/MAPK cascade. 1. Wennmann, DO. et al. (2014) Molecular biology and evolution 31, 1710-23. PMID: 24682284 2. Yu, J. et al. (2010) Developmental cell 18, 288-99. PMID: 20159598 3. Rayala, SK. et al. (2006) The Journal of biological chemistry 281, 19092-9. PMID: 16684779 4. Hilton, HN. et al. (2008) Biochimica et biophysica acta 1783, 383-93. PMID: 18190796 5. Duning, K. et al. (2008) Journal of the American Society of Nephrology : JASN 19, 1891-903. PMID: 18596123 6. Johannsen, S. et al. (2008) Neuroscience 155, 1165-73. PMID: 18672031
Note	For research use only.

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SDS-PAGE for Recombinant Human WWC1 Protein, N-His

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Datasheet

Human WWC1 Recombinant Protein (N-His).pdf

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Human WWC1 Recombinant Protein (N-His) (HV200012)

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