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Human YKT6 Recombinant Protein (N-His) (HC894012)

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Overview
Catalog No.HC894012
Description
Recombinant Human YKT6 Protein, N-His (HC894012) expressed in E. coli, spanning Met1-Met198. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionO15498
Protein lengthMet1-Met198
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 24.58 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesEC:2.3.1.-, Synaptobrevin homolog YKT6, YKT6
Background

Synaptobrevin homolog YKT6 is a ~22 kDa protein. Vesicular soluble NSF attachment protein receptor (v-SNARE) mediating vesicle docking and fusion to a specific acceptor cellular compartment. Functions in endoplasmic reticulum to Golgi transport; as part of a SNARE complex composed of GOSR1, GOSR2 and STX5. Functions in early/recycling endosome to TGN transport; as part of a SNARE complex composed of BET1L, GOSR1 and STX5. Has a S-palmitoyl transferase activity. Essential for the structural and functional organization of the Golgi apparatus.

1. McNew, JA. et al. (1997) The Journal of biological chemistry 272, 17776-83. PMID: 9211930
2. Tai, G. et al. (2004) Molecular biology of the cell 15, 4011-22. PMID: 15215310
3. Veit, M. (2004) The Biochemical journal 384, 233-7. PMID: 15479160
4. Fukasawa, M. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 4815-20. PMID: 15044687
5. Shirakawa, R. et al. (2020) The EMBO journal 39, e104120. PMID: 32128853
Note For research use only
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Formula
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