Human YTHDC1 Recombinant Protein (N-His-SUMO & C-Strep) (HD949012)

Name: Human YTHDC1 Recombinant Protein (N-His-SUMO & C-Strep)
Brand: abinScience
SKU: HD949012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HD949012
Description	Recombinant Human YTHDC1 Protein, N-His-SUMO & C-Strep (HD949012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE.. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% as determined by SDS-PAGE.
Expression system	E. coli
Accession	Q96MU7
Protein length	Ser334-Ser416
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	KIAA1966, Splicing factor YT521, YT521, YT521-B, YTH domain-containing protein 1, YTHDC1
Background	YTH domain-containing protein 1 is a ~84 kDa protein. Regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. Acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10. Specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing. In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing. 1. Xu, C. et al. (2014) Nature chemical biology 10, 927-9. PMID: 25242552 2. Xu, C. et al. (2015) The Journal of biological chemistry 290, 24902-13. PMID: 26318451 3. Xiao, W. et al. (2016) Molecular cell 61, 507-519. PMID: 26876937 4. Roundtree, IA. et al. (2017) eLife 6. PMID: 28984244 5. Zhang, Z. et al. (2010) The Journal of biological chemistry 285, 14701-10. PMID: 20167602 6. Patil, DP. et al. (2016) Nature 537, 369-373. PMID: 27602518
Note	For research use only

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Datasheet

Human YTHDC1 Recombinant Protein (N-His-SUMO & C-Strep).pdf

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Human YTHDC1 Recombinant Protein (N-His-SUMO & C-Strep) (HD949012)

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