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Recombinant Influenza A virus (H3N2) NP/Nucleoprotein Protein, C-His (VK528011)

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Recombinant Influenza A virus (H3N2) NP/Nucleoprotein Protein, C-His

Recombinant Influenza A virus (H3N2) NP/Nucleoprotein Protein, C-His

Notice · Catalog VK528011 has been discontinued.

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50ug
100ug
1mg

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Overview
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Datasheet

Overview

Catalog No.	VK528011
Description	Recombinant Influenza A virus (H3N2) NP/Nucleoprotein Protein, C-His (VK528011) expressed in Mammalian Cells, spanning Met1-Asn498. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian Cells
Accession	P16982
Protein length	Met1-Asn498
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Influenza A virus (strain A/Hong Kong/5/1983 H3N2)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Nucleoprotein, Nucleocapsid protein, Protein N, NP
Background	Nucleoprotein (NP) is a ~56 kDa protein. Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins.
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Influenza A virus (H3N2) NP/Nucleoprotein Protein, C-His.

References

Datasheet

Recombinant Influenza A virus (H3N2) NP/Nucleoprotein Protein, C-His.pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

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Concentration

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Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

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V₁ (Stock Vol.)

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Working Solution

C₂ (Working Conc.)

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V₂ (Working Vol.)

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Recombinant Influenza A virus (H3N2) NP/Nucleoprotein Protein, C-His