Lassa virus/LASV Recombinant Protein Z Protein (N-His-SUMO) (VK810012)

Name: Lassa virus/LASV Recombinant Protein Z Protein (N-His-SUMO)
Brand: abinScience
SKU: VK810012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	VK810012
Description	Recombinant Lassa virus/LASV Protein Z Protein, N-His-SUMO (VK810012) expressed in E. coli, spanning Gly2-Pro99. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O73557
Protein length	Gly2-Pro99
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Lassa virus (LASV)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	22.89 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	RING finger protein Z, Protein Z, Zinc-binding protein, Z
Background	RING finger protein Z is a ~10 kDa protein. Plays a crucial role in virion assembly and budding. Expressed late in the virus life cycle, it acts as an inhibitor of viral transcription and RNA synthesis by interacting with the viral polymerase L. Presumably recruits the NP encapsidated genome to cellular membranes at budding sites via direct interaction with NP. Plays critical roles in the final steps of viral release by interacting with host TSG101, a member of the vacuolar protein-sorting pathway and using other cellular host proteins involved in vesicle formation pathway. The budding of the virus progeny occurs after association of protein Z with the viral glycoprotein complex SSP-GP1-GP2 at the cell periphery, step that requires myristoylation of protein Z. 1. Strecker, T. et al. (2003) Journal of virology 77, 10700-5. PMID: 12970458 2. Cornu, TI. et al. (2004) Journal of virology 78, 2979-83. PMID: 14990716
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Lassa virus/LASV Protein Z Protein, N-His-SUMO

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Datasheet

Lassa virus/LASV Recombinant Protein Z Protein (N-His-SUMO).pdf

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Formula

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Lassa virus/LASV Recombinant Protein Z Protein (N-His-SUMO) (VK810012)

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References

Datasheet

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