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LCMV Glycoprotein GP1 Recombinant Protein (C-Fc) (VK012041)

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LCMV Glycoprotein GP1 Recombinant Protein (C-Fc)

LCMV Glycoprotein GP1 Recombinant Protein (C-Fc)

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Overview
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References
Datasheet
SDS

Overview

Catalog No.	VK012041
Description	Recombinant LCMV Glycoprotein GP1, C-Fc (VK012041) expressed in Mammalian Cells, spanning Met59-Ser253. Purity: >90% by SDS-PAGE. Highlights ●Fc Fusion — Enhanced stability with Protein A/G purification compatibility. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian Cells
Accession	P09991
Protein length	Met59-Ser253
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Lymphocytic choriomeningitis virus (LCMV)
Nature	Recombinant
Target	GP1
Endotoxin level	Please contact with the lab for this information.
Purity	>90%
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Pre-glycoprotein polyprotein GP complex, Pre-GP-C, Stable signal peptide, SSP, Glycoprotein G1, GP1, Glycoprotein G2, GP2, GPC
Background	Pre-glycoprotein polyprotein GP complex (GP1) is a ~56 kDa protein. Functions as a cleaved signal peptide that is retained as the third component of the GP complex (GP-C). Helps to stabilize the spike complex in its native conformation. The SSP is required for efficient glycoprotein expression, post-translational maturation cleavage of G1 and G2, glycoprotein transport to the cell surface plasma membrane, formation of infectious virus particles, and acid pH-dependent glycoprotein-mediated cell fusion. 1. Saunders, AA. et al. (2007) Journal of virology 81, 5649-57. PMID: 17376927
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant LCMV Glycoprotein GP1, C-Fc

References

Datasheet

LCMV Glycoprotein GP1 Recombinant Protein (C-Fc).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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V₁ (Stock Vol.)

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Working Solution

C₂ (Working Conc.)

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LCMV Glycoprotein GP1 Recombinant Protein (C-Fc)