LCMV RdRp/Protein L Recombinant Protein (N-His-SUMO) (VK147012)

Name: LCMV RdRp/Protein L Recombinant Protein (N-His-SUMO)
Brand: abinScience
SKU: VK147012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	VK147012
Description	Recombinant LCMV RdRp/Protein L, N-His-SUMO (VK147012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE.. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% as determined by SDS-PAGE.
Expression system	E. coli
Accession	P14240
Protein length	Asp2-Lys196
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Lymphocytic choriomeningitis virus (LCMV)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	EC:2.7.7.48, EC:3.1.-.-, L, Large structural protein, Protein L, RNA-directed RNA polymerase L, Replicase, Transcriptase, cap-snatching endonuclease
Background	RNA-directed RNA polymerase L is a ~254 kDa protein. RNA-dependent RNA polymerase, which is responsible for the replication and transcription of the viral RNA genome using antigenomic RNA as an intermediate. During transcription, synthesizes subgenomic RNAs and assures their capping by a cap-snatching mechanism, which involves the endonuclease activity cleaving the host capped pre-mRNAs. These short capped RNAs are then used as primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. 1. Liu, L. et al. (2023) Protein & cell 14, 703-707. PMID: 37038286
Note	For research use only

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Datasheet

LCMV RdRp/Protein L Recombinant Protein (N-His-SUMO).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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LCMV RdRp/Protein L Recombinant Protein (N-His-SUMO) (VK147012)

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References

Datasheet

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