MeV Phosphoprotein Recombinant Protein (N-His) (VK796012)

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Overview

Catalog No.	VK796012
Description	Recombinant MeV Phosphoprotein, N-His (VK796012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE.. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% as determined by SDS-PAGE.
Expression system	E. coli
Accession	Q9WMB4
Protein length	Gly304-Leu377
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Measles virus (strain Ichinose-B95a) (MeV)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	P/V, Phosphoprotein, Protein P
Background	Phosphoprotein is a ~53 kDa protein. Essential cofactor of the RNA polymerase L that plays a central role in the transcription and replication by forming the polymerase complex with RNA polymerase L and recruiting L to the genomic N-RNA template for RNA synthesis. Also plays a central role in the encapsidation of nascent RNA chains by forming the encapsidation complex with the nucleocapsid protein N (N-P complex). Acts as a chaperone for newly synthesized free N protein, so-called N0, allowing encapsidation of nascent RNA chains during replication. The nucleoprotein protein N prevents excessive phosphorylation of P, which leads to down-regulation of viral transcription/ replication. Participates, together with N, in the formation of viral factories (viroplasms), which are large inclusions in the host cytoplasm where replication takes place. 1. Guseva, S. et al. (2020) Science advances 6, eaaz7095. PMID: 32270045
Note	For research use only

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SDS-PAGE for Recombinant MeV P/Phosphoprotein Protein, N-His

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Datasheet

MeV Phosphoprotein Recombinant Protein (N-His).pdf

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MeV Phosphoprotein Recombinant Protein (N-His) (VK796012)

Overview

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References

Datasheet

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