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MojV Matrix Recombinant Protein (N-His) (VK499032)

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Overview
Catalog No.VK499032
Description
Recombinant Mojiang virus/MojV Matrix Protein, N-His (VK499032) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% as determined by SDS-PAGE.
Expression systemE. coli
AccessionYP_009094093.1
Protein lengthMet1-Asn340
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesMojiang virus (MojV)
Nature Recombinant
Endotoxin level Remove endotoxin and sterilization is required for cell assay.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 40.13 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Matrix protein is a ~39 kDa protein. Plays a crucial role in virion assembly and budding. Forms a shell at the inner face of the plasma membrane. Transits through the host nucleus before gaining the functional ability to localize and bud from the plasma membrane. Mediates together with fusion protein the incorporation of the glycoprotein to the viral particles. Also participates in the inhibition of the host interferon type I antiviral response by interacting with and thereby inhibiting host TRIM6.

1. Ciancanelli, MJ. et al. (2006) Journal of virology 80, 12070-8. PMID: 17005661
2. Patch, JR. et al. (2007) Virology journal 4, 1. PMID: 17204159
3. Patch, JR. et al. (2008) Virology journal 5, 137. PMID: 19000317
4. Wang, YE. et al. (2010) PLoS pathogens 6, e1001186. PMID: 21085610
5. Johnston, GP. et al. (2017) Journal of virology 91. PMID: 28250132
6. Bharaj, P. et al. (2016) PLoS pathogens 12, e1005880. PMID: 27622505
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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