Recombinant Mouse ACE2 Protein, C-His (MV157011) expressed in Mammalian Cells, spanning Gln18-Thr740. Purity: >90% by SDS-PAGE.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
Expression system
Mammalian cells
Accession
Q8R0I0
Protein length
Gln18-Thr740
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Mus musculus (Mouse)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
86.38 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Angiotensin-converting enzyme 2 is a ~92 kDa protein. Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis. Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II. Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin. Also cleaves other biological peptides, such as apelins, casomorphins and dynorphin A. By cleavage of angiotensin II, may be an important regulator of heart function.
2. Donoghue, M. et al. (2003) Journal of molecular and cellular cardiology 35, 1043-53. PMID: 12967627
3. Imai, Y. et al. (2005) Nature 436, 112-6. PMID: 16001071
4. Kowalczuk, S. et al. (2008) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 22, 2880-7. PMID: 18424768
5. Camargo, SM. et al. (2009) Gastroenterology 136, 872-82. PMID: 19185582
6. Fairweather, SJ. et al. (2012) The Biochemical journal 446, 135-48. PMID: 22677001
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