Mouse ADRB2 Recombinant Protein (N-His-KSI) (MY357012)

Name: Mouse ADRB2 Recombinant Protein (N-His-KSI)
Brand: abinScience
SKU: MY357012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	MY357012
Description	Recombinant Mouse ADRB2 Protein, N-His-KSI (MY357012) expressed in E. coli, spanning Pro330-Leu418. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P18762
Protein length	Pro330-Leu418
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	25.52 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Beta-2 adrenergic receptor; Beta-2 adrenoreceptor; Beta-2 adrenoceptor; Adrb2; Adrb2r
Background	Beta-2 adrenergic receptor is a ~46 kDa protein. G protein-coupled receptor for catecholamines that couples to both G(s) and G(i) proteins, activating bifurcated signaling pathways. ADRB2 binds epinephrine (Epi) with an approximately 30-fold greater affinity than norepinephrine (NE). In the heart, Epi- and NE-activated ADRB2 induces rapid and slow cardiomyocyte contraction rate, respectively. Both NE and Epi promote coupling to G(s)/PKA pathway to regulate myocyte contraction rate. Epi also promotes ADRB2 coupling to G(i) proteins to exert cardioprotective effects especially in the conditions of hypoxia and oxidative stress through the G(i)/PI3K/Akt signaling pathway. 1. Zhu, WZ. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 1607-12. PMID: 11171998 2. Wang, Y. et al. (2008) The Journal of biological chemistry 283, 1799-807. PMID: 18056263 3. Allen, JM. et al. (1988) The EMBO journal 7, 133-8. PMID: 2834198
Note	For research use only

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SDS-PAGE for Recombinant Mouse ADRB2 Protein, N-His-KSI

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Datasheet

Mouse ADRB2 Recombinant Protein (N-His-KSI).pdf

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Mouse ADRB2 Recombinant Protein (N-His-KSI) (MY357012)

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