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Mouse ANGPTL8 Recombinant Protein (N-His) (MV410012)

Mouse ANGPTL8 Recombinant Protein (N-His)
Mouse ANGPTL8 Recombinant Protein (N-His)
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Overview
Catalog No.MV410012
Description
Recombinant Mouse ANGPTL8 Protein, N-His (MV410012) expressed in E. coli, spanning Val16-Ala198. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionQ8R1L8
Protein lengthVal16-Ala198
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesMus musculus (Mouse)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 23.39 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesAngiopoietin-like protein 8, Betatrophin, Lipasin, Refeeding-induced fat and liver protein, Angptl8, Gm6484, Rifl
Background

Angiopoietin-like protein 8 (ANGPTL8) is a ~22 kDa protein. Hormone that acts as a blood lipid regulator by regulating serum triglyceride levels. May be involved in the metabolic transition between fasting and refeeding: required to direct fatty acids to adipose tissue for storage in the fed state. According to a report, may act by promoting ANGPTL3 cleavage. According to another study, not required for cleavage of ANGPTL3.

1. Ren, G. et al. (2012) American journal of physiology. Endocrinology and metabolism 303, E334-51. PMID: 22569073
2. Zhang, R. (2012) Biochemical and biophysical research communications 424, 786-92. PMID: 22809513
3. Quagliarini, F. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 19751-6. PMID: 23150577
4. Wang, Y. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 16109-14. PMID: 24043787
Note For research use only.
Images
  • Mouse ANGPTL8 Recombinant Protein (N-His)

    SDS-PAGE

    SDS-PAGE for Recombinant Mouse ANGPTL8 Protein, N-His

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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