●Fc Fusion — Enhanced stability with Protein A/G purification compatibility.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
Expression system
Mammalian cells
Accession
P15261
Protein length
Ala26-Arg251
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Mus musculus (Mouse)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Predicted molecular weight
53.94 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Interferon gamma receptor 1 is a ~52 kDa protein. Receptor subunit for interferon gamma/INFG that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (, PubMed:20926559, PubMed:27286456). Associates with transmembrane accessory factor IFNGR2 to form a functional receptor. Upon ligand binding, the intracellular domain of IFNGR1 opens out to allow association of downstream signaling components JAK1 and JAK2. In turn, activated JAK1 phosphorylates IFNGR1 to form a docking site for STAT1. Subsequent phosphorylation of STAT1 leads to its dimerization, translocation to the nucleus, and stimulation of target gene transcription.
1. Kolokoltsova, OA. et al. (2010) Journal of virology 84, 13063-7. PMID: 20926559
2. Zhang, C. et al. (2016) Oncotarget 7, 42099-42109. PMID: 27286456
3. Cofano, F. et al. (1990) The Journal of biological chemistry 265, 4064-71. PMID: 2137461
4. Kumar, CS. et al. (1989) The Journal of biological chemistry 264, 17939-46. PMID: 2530216
5. Gray, PW. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 8497-501. PMID: 2530582
6. Munro, S. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 9248-52. PMID: 2531896
7. Hemmi, S. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 9901-5. PMID: 2532365
8. Wei, B. et al. (2010) American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 10, 69-80. PMID: 19889125
9. Qing, Y. et al. (2004) The Journal of biological chemistry 279, 41679-85. PMID: 15284232
10. Qing, Y. et al. (2005) The Journal of biological chemistry 280, 1849-53. PMID: 15522878
Note
For research use only
Images
SDS-PAGE
SDS-PAGE for Recombinant Mouse CD119/IFNGR1 Protein, C-Fc
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