Mouse CD122/IL2RB Recombinant Protein (C-Fc) (MB912011)

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Overview

Catalog No.	MB912011
Description	Recombinant Mouse CD122/IL2RB Protein, C-Fc (MB912011) expressed in Mammalian Cells, spanning Ala27-Glu240. Highlights ●Fc Fusion — Enhanced stability with Protein A/G purification compatibility. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian Cells
Accession	P16297
Protein length	Ala27-Glu240
Applications	SDS-PAGE, WB, ELISA, Immunogen, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Target	CD122
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Interleukin-2 receptor subunit beta, IL-2 receptor subunit beta, IL-2R subunit beta, IL-2RB, High affinity IL-2 receptor subunit beta, Interleukin-15 receptor subunit beta, p70-75, p75, CD122, IL2RB
Background	Interleukin-2 receptor subunit beta (CD122) is a ~60 kDa protein. Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15.
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Mouse CD122/IL2RB Protein, C-Fc.

References

MB912011-SDS-PAGE-1.jpg

Datasheet

Mouse CD122/IL2RB Recombinant Protein (C-Fc).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Mouse CD122/IL2RB Recombinant Protein (C-Fc) (MB912011)

Overview

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References

Datasheet

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