ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Mus musculus (Mouse)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
23.66 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
SLAM family member 4, SLAMF4, h2B4, Signaling lymphocytic activation molecule 4, Natural killer cell receptor 2B4, NKR2B4, NAIL, NK cell type I receptor protein 2B4, CD244, NK cell activation-inducing ligand, 2B4
Background
Natural killer cell receptor 2B4 (CD244) is a ~44 kDa protein. Heterophilic receptor of the signaling lymphocytic activation molecule (SLAM) family; its ligand is CD48. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Acts as activating natural killer (NK) cell receptor. Activating function implicates association with SH2D1A and FYN.
1. Lee, KM. et al. (2003) Journal of immunology (Baltimore, Md. : 1950) 170, 4881-5. PMID: 12734329
2. Dong, Z. et al. (2009) Nature immunology 10, 973-80. PMID: 19648922
3. Wang, N. et al. (2010) Journal of immunology (Baltimore, Md. : 1950) 185, 5683-7. PMID: 20962259
4. Garni-Wagner, BA. et al. (1993) Journal of immunology (Baltimore, Md. : 1950) 151, 60-70. PMID: 8326140
5. Chen, R. et al. (2004) Molecular and cellular biology 24, 5144-56. PMID: 15169881
6. Bloch-Queyrat, C. et al. (2005) The Journal of experimental medicine 202, 181-92. PMID: 15998796
7. Dong, Z. et al. (2012) Immunity 36, 974-85. PMID: 22683124
8. Lee, KM. et al. (2006) Blood 107, 3181-8. PMID: 15905190
9. Roncagalli, R. et al. (2005) Nature immunology 6, 1002-10. PMID: 16127454
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Mouse CD244 Protein, N-His
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