ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Mus musculus (Mouse)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
15.10 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
CMRF35-like molecule 1 (CD300f/CD300LF) is a ~36 kDa protein. Acts as an inhibitory receptor for myeloid cells and mast cells. Positively regulates the phagocytosis of apoptotic cells (efferocytosis) via phosphatidylserine (PS) recognition; recognizes and binds PS as a ligand which is expressed on the surface of apoptotic cells. Plays an important role in the maintenance of immune homeostasis, by promoting macrophage-mediated efferocytosis and by inhibiting dendritic cell-mediated efferocytosis. Negatively regulates Fc epsilon receptor-dependent mast cell activation and allergic responses via binding to ceramide which acts as a ligand. May act as a coreceptor for interleukin 4 (IL-4).
1. Izawa, K. et al. (2007) The Journal of biological chemistry 282, 17997-18008. PMID: 17438331
2. Choi, SC. et al. (2011) Journal of immunology (Baltimore, Md. : 1950) 187, 3483-7. PMID: 21865548
3. Tian, L. et al. (2016) Cell death and differentiation 23, 1086-96. PMID: 26768664
4. Izawa, K. et al. (2012) Immunity 37, 827-39. PMID: 23123064
5. Moshkovits, I. et al. (2015) Proceedings of the National Academy of Sciences of the United States of America 112, 8708-13. PMID: 26124135
6. Chung, DH. et al. (2003) Journal of immunology (Baltimore, Md. : 1950) 171, 6541-8. PMID: 14662855
7. Can, I. et al. (2008) Journal of immunology (Baltimore, Md. : 1950) 180, 207-13. PMID: 18097021
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Mouse CD300f/CD300LF Protein, N-His
中文
English
한국어
日本語
Español
Français
Русский
