Mouse Desmoglein-3 Recombinant Protein (N-His) (MW575012)

Name: Mouse Desmoglein-3 Recombinant Protein (N-His)
Brand: abinScience
SKU: MW575012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	MW575012
Description	Recombinant Mouse DSG3 Protein, N-His (MW575012) expressed in E. coli, spanning Asn151-Asp591. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O35902
Protein length	Asn151-Asp591
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Mus musculus (Mouse)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	51.14 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Desmoglein-3, 130 kDa pemphigus vulgaris antigen homolog, Dsg3
Background	Desmoglein-3 (DSG3) is a ~107 kDa protein. A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion. Required for adherens and desmosome junction assembly in response to mechanical force in keratinocytes. Required for desmosome-mediated cell-cell adhesion of cells surrounding the telogen hair club and the basal layer of the outer root sheath epithelium, consequently is essential for the anchoring of telogen hairs in the hair follicle. Required for the maintenance of the epithelial barrier via promoting desmosome-mediated intercellular attachment of suprabasal epithelium to basal cells. May play a role in the protein stability of the desmosome plaque components DSP, JUP, PKP1, PKP2 and PKP3. 1. Koch, PJ. et al. (1997) The Journal of cell biology 137, 1091-102. PMID: 9166409 2. Koch, PJ. et al. (1998) Journal of cell science 111 ( Pt 17), 2529-37. PMID: 9701552 3. Rötzer, V. et al. (2016) The Journal of investigative dermatology 136, 301-10. PMID: 26763450
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Mouse DSG3 Protein, N-His

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Datasheet

Mouse Desmoglein-3 Recombinant Protein (N-His).pdf

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Formula

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Mouse Desmoglein-3 Recombinant Protein (N-His) (MW575012)

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